ISG20 monoclonal antibody (M01), clone 1B2-3C9
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ISG20.
Immunogen
ISG20 (AAH07922.1, 1 a.a. ~ 181 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAGSREVVAMDCEMVGLGPHRESGLARCSLVNVHGAVLYDKFIRPEGEITDYRTRVSGVTPQHMVGATPFAVARLEILQLLKGKLVVGHDLKHDFQALKEDMSGYTIYDTSTDRLLWREAKLDHCRRVSLRVLSERLLHKSIQNSLLGHSSVEDARATMELYQISQRIRARRGLPRLAVSD
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (45.65 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ISG20 expression in transfected 293T cell line by ISG20 monoclonal antibody (M01), clone 1B2-3C9.
Lane 1: ISG20 transfected lysate(20.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ISG20 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to ISG20 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — ISG20
Entrez GeneID
3669GeneBank Accession#
BC007922Protein Accession#
AAH07922.1Gene Name
ISG20
Gene Alias
CD25, HEM45
Gene Description
interferon stimulated exonuclease gene 20kDa
Omim ID
604533Gene Ontology
HyperlinkOther Designations
interferon stimulated exonuclease|interferon stimulated gene 20kDa
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Interactome
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Publication Reference
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Antiviral activities of ISG20 in positive-strand RNA virus infections.
Zhou Z, Wang N, Woodson SE, Dong Q, Wang J, Liang Y, Rijnbrand R, Wei L, Nichols JE, Guo JT, Holbrook MR, Lemon SM, Li K.
Virology 2011 Jan; 409(2):175.
Application:WB-Tr, Human, Huh7.5 cells.
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Intracellular activation of interferon regulatory factor-1 by nanobodies to the multi-functional (Mf1) domain.
Moeller A, Pion E, Narayan V, Ball KL.
The Journal of Biological Chemistry 2010 Dec; 285(49):38348.
Application:WB-Tr, Human, HeLa cells.
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Antiviral activities of ISG20 in positive-strand RNA virus infections.
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