IRF5 monoclonal antibody (M03), clone 1H6
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More Files
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Western Blot (Cell lysate)
IRF5 monoclonal antibody (M03), clone 1H6. Western Blot analysis of IRF5 expression in NIH/3T3(Cat # L018V1 ).
Western Blot (Cell lysate)
IRF5 monoclonal antibody (M03), clone 1H6 Western Blot analysis of IRF5 expression in A-431 ( Cat # L015V1 ).
Immunoprecipitation
Immunoprecipitation of IRF5 transfected lysate using anti-IRF5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRF5 MaxPab mouse polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IRF5 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to IRF5 on A-431 cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.84 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant IRF5.
Immunogen
IRF5 (NP_002191, 395 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
TPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
IRF5 monoclonal antibody (M03), clone 1H6. Western Blot analysis of IRF5 expression in NIH/3T3(Cat # L018V1 ).Western Blot (Cell lysate)
IRF5 monoclonal antibody (M03), clone 1H6 Western Blot analysis of IRF5 expression in A-431 ( Cat # L015V1 ).Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of IRF5 transfected lysate using anti-IRF5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRF5 MaxPab mouse polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IRF5 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to IRF5 on A-431 cell. [antibody concentration 10 ug/ml] -
Gene Info — IRF5
Entrez GeneID
3663GeneBank Accession#
NM_002200Protein Accession#
NP_002191Gene Name
IRF5
Gene Alias
SLEB10
Gene Description
interferon regulatory factor 5
Omim ID
607218Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist. [provided by RefSeq
Other Designations
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