IRF4 monoclonal antibody (M05), clone 2D1
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant IRF4.
Immunogen
IRF4 (NP_002451, 342 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (90)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M05), clone 2D1.
Lane 1: IRF4 transfected lysate(51.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IRF4 is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of IRF4 over-expressed 293 cell line, cotransfected with IRF4 Validated Chimera RNAi ( Cat # H00003662-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRF4 monoclonal antibody (M05), clone 2D1 (Cat # H00003662-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to IRF4 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — IRF4
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Interactome
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Disease
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