IDO1 monoclonal antibody (M05J), clone 4D2
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More Files
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Western Blot (Cell lysate)
IDO1 monoclonal antibody (M05J), clone 4D2. Western Blot analysis of IDO1 expression in HeLa.
Western Blot (Transfected lysate)
Western Blot analysis of IDO1 expression in transfected 293T cell line by IDO1 monoclonal antibody (M05J), clone 4D2.
Lane 1: IDO1 transfected lysate (Predicted MW: 45.3 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to IDO1 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IDO1 is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (70.07 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant IDO1.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
IDO1 (AAH27882.1, 1 a.a. ~ 403 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG
Host
Mouse
Reactivity
Human
Preparation Method
Cell Culture Production
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (70.07 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
IDO1 monoclonal antibody (M05J), clone 4D2. Western Blot analysis of IDO1 expression in HeLa.Western Blot (Transfected lysate)
Western Blot analysis of IDO1 expression in transfected 293T cell line by IDO1 monoclonal antibody (M05J), clone 4D2.
Lane 1: IDO1 transfected lysate (Predicted MW: 45.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to IDO1 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IDO1 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — IDO1
Entrez GeneID
3620GeneBank Accession#
BC027882.1Protein Accession#
AAH27882.1Gene Name
IDO1
Gene Alias
CD107B, IDO, INDO
Gene Description
indoleamine 2,3-dioxygenase 1
Omim ID
147435Gene Ontology
HyperlinkGene Summary
Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.[supplied by OMIM
Other Designations
indole 2,3-dioxygenase|indoleamine-pyrrole 2,3 dioxygenase
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Interactome
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Pathway
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Disease
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