Western blot analysis of IMPDH2 over-expressed 293 cell line, cotransfected with IMPDH2 Validated Chimera RNAi ( Cat # H00003615-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IMPDH2 monoclonal antibody (M01), clone 1E12-B6 (Cat # H00003615-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq