IMPDH2 monoclonal antibody (M01), clone 1E12-B6
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant IMPDH2.
Immunogen
IMPDH2 (AAH12840, 1 a.a. ~ 514 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MADYLISGGTSYVPDDGLTAQQLFNCGDGLTYNDFLILPGYIDFTADQVDLTSALTKKITLKTPLVSSPMDTVTEAGMAIAMALTGGIGFIHHNCTPEFQANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVIGGNVVTAAQAKNLIDAGVDALRVGMGSGSICITQEVLACGRPQATAVYKVSEYARRFGVPVIADGGIQNVGHIAKALALGASTVMMGSLLAATTEAPGEYFFSDGIRLKKYRGMGSLDAMDKHLSSQNRYFSEADKIKVAQGVSGAVQDKGSIHKFVPYLIAGIQHSCQDIGAKSLTQVRAMMYSGELKFEKRTSSAQVEGGVHSLHSYEKRLF
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of IMPDH2 expression in transfected 293T cell line by IMPDH2 monoclonal antibody (M01), clone 1E12-B6.
Lane 1: IMPDH2 transfected lysate(55.8 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of IMPDH2 transfected lysate using anti-IMPDH2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IMPDH2 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IMPDH2 is approximately 0.3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of IMPDH2 over-expressed 293 cell line, cotransfected with IMPDH2 Validated Chimera RNAi ( Cat # H00003615-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IMPDH2 monoclonal antibody (M01), clone 1E12-B6 (Cat # H00003615-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — IMPDH2
Entrez GeneID
3615GeneBank Accession#
BC012840Protein Accession#
AAH12840Gene Name
IMPDH2
Gene Alias
IMPD2, IMPDH-II
Gene Description
IMP (inosine monophosphate) dehydrogenase 2
Omim ID
146691Gene Ontology
HyperlinkGene Summary
This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq
Other Designations
IMP dehydrogenase 2|IMP oxireductase 2|inosine 5' phosphate dehydrogenase 2|inosine monophosphate dehydrogenase 2|inosine monophosphate dehydrogenase type II|inosine monophosphate oxireductase 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Proteomic analysis of the effects of the immunomodulatory mycotoxin deoxynivalenol.
da Costa AN, Mijal RS, Keen JN, Findlay JB, Wild CP.
Proteomics 2011 May; 11(10):1903.
Application:Flow Cyt, Human, RPMI 1788, Jurkat E6.1 cells.
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Proteomic analysis of the effects of the immunomodulatory mycotoxin deoxynivalenol.
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