ILF3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ILF3 full-length
Host
Human
Theoretical MW (kDa)
76
Interspecies Antigen Sequence
Mouse (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ILF3 antibody (H00003609-B01P) by Western Blots.
SDS-PAGE Gel
ILF3 transfected lysate.
Western Blot
Lane 1: ILF3 transfected lysate ( 76.00 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ILF3
Entrez GeneID
3609GeneBank Accession#
NM_004516Protein Accession#
NP_004507Gene Name
ILF3
Gene Alias
CBTF, DRBF, DRBP76, MMP4, MPHOSPH4, MPP4, NF-AT-90, NF110, NF90, NFAR, NFAR-1, NFAR2, TCP110, TCP80
Gene Description
interleukin enhancer binding factor 3, 90kDa
Omim ID
603182Gene Ontology
HyperlinkGene Summary
This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms
Other Designations
M-phase phosphoprotein 4|double-stranded RNA-binding protein, 76 kD|dsRNA binding protein NFAR-2/MPP4|interleukin enhancer binding factor 3|nuclear factor associated with dsRNA|nuclear factor of activated T-cells, 90 kD|translational control protein 80
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Interactome
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Disease
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