IL5RA 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-IL5RA full-length
Host
Human
Theoretical MW (kDa)
38
Interspecies Antigen Sequence
Mouse (72); Rat (71)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-IL5RA antibody (H00003568-B01P) by Western Blots.
SDS-PAGE Gel
IL5RA transfected lysate.
Western Blot
Lane 1: IL5RA transfected lysate ( 38 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — IL5RA
Entrez GeneID
3568GeneBank Accession#
NM_175725.1Protein Accession#
NP_783852.1Gene Name
IL5RA
Gene Alias
CD125, CDw125, HSIL5R3, IL5R, MGC26560
Gene Description
interleukin 5 receptor, alpha
Omim ID
147851Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. [provided by RefSeq
Other Designations
CD125 antigen|OTTHUMP00000115257|OTTHUMP00000115373|interleukin 5 receptor type 3|interleukin-5 receptor alpha chain|interleukin-5 receptor alpha subunit
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Interactome
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Disease
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