IL3RA 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-IL3RA full-length
Host
Human
Theoretical MW (kDa)
43.3
Interspecies Antigen Sequence
Mouse (31); Rat (31)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-IL3RA antibody (H00003563-B01) by Western Blots.
SDS-PAGE Gel
IL3RA transfected lysate.
Western Blot
Lane 1: IL3RA transfected lysate ( 43.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — IL3RA
Entrez GeneID
3563GeneBank Accession#
NM_002183.2Protein Accession#
NP_002174.1Gene Name
IL3RA
Gene Alias
CD123, IL3R, IL3RAY, IL3RX, IL3RY, MGC34174, hIL-3Ra
Gene Description
interleukin 3 receptor, alpha (low affinity)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. [provided by RefSeq
Other Designations
CD123 antigen|Interleukin-3 receptor|OTTHUMP00000022828|interleukin 3 receptor, alpha
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Interactome
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