IFNG 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-IFNG full-length
Host
Human
Theoretical MW (kDa)
18.26
Interspecies Antigen Sequence
Mouse (41); Rat (39)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-IFNG antibody (H00003458-B01) by Western Blots.
SDS-PAGE Gel
IFNG transfected lysate
Western Blot
Lane 1: IFNG transfected lysate ( 18.26 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — IFNG
Entrez GeneID
3458GeneBank Accession#
NM_000619Protein Accession#
NP_000610Gene Name
IFNG
Gene Alias
IFG, IFI
Gene Description
interferon, gamma
Gene Ontology
HyperlinkGene Summary
Interferon-gamma (IFNG), or type II interferon, is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNG in the immune system stems in part from its ability to inhibit viral replication directly, but most importantly derives from its immunostimulatory and immunomodulatory effects. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 (MIM 186940) and CD8 (see MIM 186910) cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops (Schoenborn and Wilson, 2007 [PubMed 17981204]).[supplied by OMIM
Other Designations
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Interactome
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Pathway
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