IDS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
IDS transfected lysate.
Western Blot
Lane 1: IDS transfected lysate ( 60.61 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-IDS full-length
Host
Human
Theoretical MW (kDa)
60.61
Interspecies Antigen Sequence
Mouse (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-IDS antibody (H00003423-B01) by Western Blots.
SDS-PAGE Gel
IDS transfected lysate.
Western Blot
Lane 1: IDS transfected lysate ( 60.61 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — IDS
Entrez GeneID
3423GeneBank Accession#
NM_000202.2Protein Accession#
NP_000193.1Gene Name
IDS
Gene Alias
MPS2, SIDS
Gene Description
iduronate 2-sulfatase
Omim ID
309900Gene Ontology
HyperlinkGene Summary
Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. A splice variant of this gene has been described. [provided by RefSeq
Other Designations
Hunter syndrome|OTTHUMP00000024207|OTTHUMP00000024210|alpha-L-iduronate sulfate sulfatase|iduronate-2-sulfatase|idursulfase
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Interactome
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Pathway
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Disease
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