IDI1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
IDI1 transfected lysate.
Western Blot
Lane 1: IDI1 transfected lysate ( 25.19 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-IDI1 full-length
Host
Human
Theoretical MW (kDa)
25.19
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-IDI1 antibody (H00003422-B01) by Western Blots.
SDS-PAGE Gel
IDI1 transfected lysate.
Western Blot
Lane 1: IDI1 transfected lysate ( 25.19 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — IDI1
Entrez GeneID
3422GeneBank Accession#
BC057827.1Protein Accession#
AAH57827.1Gene Name
IDI1
Gene Alias
IPP1, IPPI1
Gene Description
isopentenyl-diphosphate delta isomerase 1
Omim ID
604055Gene Ontology
HyperlinkGene Summary
IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity. [provided by RefSeq
Other Designations
IPP isomerase|OTTHUMP00000018951|isopentenyl diphosphate dimethylallyl diphosphate isomerase 1|isopentenyl pyrophosphate isomerase 1|isopentenyl-diphosphate delta isomerase
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Interactome
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Pathway
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