IDI1 monoclonal antibody (M01), clone 6G10
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant IDI1.
Immunogen
IDI1 (NP_004499, 175 a.a. ~ 283 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
IPLEEVPPEEINYLTRIHYKAQSDGIWGEHEIDYILLVRKNVTLNPDPNEIKSYCYVSKEELKELLKKAASGEIKITPWFKIIAATFLFKWWDNLNHLNQFVDHEKIYR
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
IDI1 monoclonal antibody (M01), clone 6G10 Western Blot analysis of IDI1 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of IDI1 expression in transfected 293T cell line by IDI1 monoclonal antibody (M01), clone 6G10.
Lane 1: IDI1 transfected lysate(26.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IDI1 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — IDI1
Entrez GeneID
3422GeneBank Accession#
NM_004508Protein Accession#
NP_004499Gene Name
IDI1
Gene Alias
IPP1, IPPI1
Gene Description
isopentenyl-diphosphate delta isomerase 1
Omim ID
604055Gene Ontology
HyperlinkGene Summary
IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity. [provided by RefSeq
Other Designations
IPP isomerase|OTTHUMP00000018951|isopentenyl diphosphate dimethylallyl diphosphate isomerase 1|isopentenyl pyrophosphate isomerase 1|isopentenyl-diphosphate delta isomerase
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Interactome
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Pathway
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