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IDH3B 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003420-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-IDH3B full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 42.2
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-IDH3B antibody (H00003420-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00003420-T01
    IDH3B transfected lysate.
    Western Blot
    QC Testing of H00003420-T01
    Lane 1: IDH3B transfected lysate ( 42.20 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3420
  • Gene Name:
  • IDH3B
  • Gene Alias:
  • FLJ11043,H-IDHB,MGC903
  • Gene Description:
  • isocitrate dehydrogenase 3 (NAD+) beta
  • Gene Summary:
  • Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq
  • Other Designations:
  • NAD+-specific ICDH,NAD+-specific isocitrate dehydrogenase b subunit,NAD+-specific isocitrate dehydrogenase beta,OTTHUMP00000030023,OTTHUMP00000030024,isocitrate dehydrogenase 3, beta subunit,isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, beta s
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