ID4 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ID4 full-length
Host
Human
Theoretical MW (kDa)
17.82
Interspecies Antigen Sequence
Mouse (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ID4 antibody (H00003400-B01) by Western Blots.
SDS-PAGE Gel
ID4 transfected lysate.
Western Blot
Lane 1: ID4 transfected lysate ( 17.82 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ID4
Entrez GeneID
3400GeneBank Accession#
NM_001546Protein Accession#
NP_001537Gene Name
ID4
Gene Alias
IDB4, bHLHb27
Gene Description
inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
Omim ID
600581Gene Ontology
HyperlinkGene Summary
Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM
Other Designations
OTTHUMP00000016081
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