ID4 293T Cell Transient Overexpression Lysate(Denatured)

Catalog # H00003400-T01

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Size:100 uL
Price: USD $ 247.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
SDS-PAGE Gel
QC Test

SDS-PAGE Gel

ID4 transfected lysate.

Western Blot
QC Test

Western Blot

Lane 1: ID4 transfected lysate ( 17.82 KDa)
Lane 2: Non-transfected lysate.

  • Specification

    Transfected Cell Line

    293T

    Plasmid

    pCMV-ID4 full-length

    Host

    Human

    Theoretical MW (kDa)

    17.82

    Interspecies Antigen Sequence

    Mouse (98)

    Quality Control Testing

    Transient overexpression cell lysate was tested with Anti-ID4 antibody (H00003400-B01) by Western Blots.

    SDS-PAGE Gel

    ID4 transfected lysate.

    Western Blot

    Lane 1: ID4 transfected lysate ( 17.82 KDa)
    Lane 2: Non-transfected lysate.

    Storage Buffer

    1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

  • Gene Info — ID4

    Entrez GeneID

    3400

    GeneBank Accession#

    NM_001546

    Protein Accession#

    NP_001537

    Gene Name

    ID4

    Gene Alias

    IDB4, bHLHb27

    Gene Description

    inhibitor of DNA binding 4, dominant negative helix-loop-helix protein

    Omim ID

    600581

    Gene Ontology

    Hyperlink

    Gene Summary

    Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM

    Other Designations

    OTTHUMP00000016081

  • Interactome
  • Pathway
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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