ID3 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ID3 full-length
Host
Human
Theoretical MW (kDa)
13.2
Interspecies Antigen Sequence
Mouse (96); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ID3 antibody (H00003399-B01) by Western Blots.
Western Blot
Lane 1: ID3 transfected lysate ( 13.09 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
ID3 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ID3
Entrez GeneID
3399GeneBank Accession#
BC003107Protein Accession#
AAH03107Gene Name
ID3
Gene Alias
HEIR-1, bHLHb25
Gene Description
inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
Omim ID
600277Gene Ontology
HyperlinkGene Summary
Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.[supplied by OMIM
Other Designations
OTTHUMP00000003263|inhibitor of DNA binding 3
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