Western blot analysis of ID3 over-expressed 293 cell line, cotransfected with ID3 Validated Chimera RNAi ( Cat # H00003399-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ID3 monoclonal antibody (M01), clone 4G1 (Cat # H00003399-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.[supplied by OMIM