ID3 monoclonal antibody (M01), clone 4G1
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ID3.
Immunogen
ID3 (NP_002158, 1 a.a. ~ 83 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKALSPVRGCYEAVCCLSERSLAIARGRGKGPAAEEPLSLLDDMNHCYSRLRELVPGVPRGTQLSQVEILQRVIDYILDLQVV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (98)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (34.87 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ID3 is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of ID3 over-expressed 293 cell line, cotransfected with ID3 Validated Chimera RNAi ( Cat # H00003399-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ID3 monoclonal antibody (M01), clone 4G1 (Cat # H00003399-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — ID3
Entrez GeneID
3399GeneBank Accession#
NM_002167Protein Accession#
NP_002158Gene Name
ID3
Gene Alias
HEIR-1, bHLHb25
Gene Description
inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
Omim ID
600277Gene Ontology
HyperlinkGene Summary
Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.[supplied by OMIM
Other Designations
OTTHUMP00000003263|inhibitor of DNA binding 3
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Interactome
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Disease
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