Western blot analysis of ID1 over-expressed 293 cell line, cotransfected with ID1 Validated Chimera RNAi ( Cat # H00003397-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ID1 monoclonal antibody (M02), clone 1F7 (Cat # H00003397-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
DNA-binding protein inhibitor ID-1,OTTHUMP00000030534,OTTHUMP00000030535,dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein),inhibitor of DNA binding 1,inhibitor of differentiation 1