ICAM1 monoclonal antibody (M01), clone 3H8-2G6
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ICAM1.
Immunogen
ICAM1 (AAH15969, 1 a.a. ~ 532 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAPSSPRPALPALLVLLGALFPGPGNAQTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKTFLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRRDHHGANFSCRTELDLRPQGLELFENTSAPYQLQTFVLPATPPQLVSPRVLEVDTQGTVVCSLDGLFPVSEAQVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTYLRRARSTQGEVTRKVAVNVLSPRYEIVIITVVAAAVIMGTAGLSTYLYNRQRKIKKYRLQQAQKGTPMKPNTQATPP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (53); Rat (51)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (84.26 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ICAM1 expression in transfected 293T cell line by ICAM1 monoclonal antibody (M01), clone 3H8-2G6.
Lane 1: ICAM1 transfected lysate (Predicted MW: 57.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of ICAM1 transfected lysate using anti-ICAM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ICAM1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ICAM1 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — ICAM1
Entrez GeneID
3383GeneBank Accession#
BC015969Protein Accession#
AAH15969Gene Name
ICAM1
Gene Alias
BB2, CD54, P3.58
Gene Description
intercellular adhesion molecule 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq
Other Designations
cell surface glycoprotein P3.58|human rhinovirus receptor|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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Lipid-Induced Toxicity Stimulates Hepatocytes to Release Angiogenic Microparticles That Require Vanin-1 for Uptake by Endothelial Cells.
Povero D, Eguchi A, Niesman IR, Andronikou N, de Mollerat du Jeu X, Mulya A, Berk M, Lazic M, Thapaliya S, Parola M, Patel HH, Feldstein AE.
Science Signaling 2013 Oct; 6(296):ra88.
Application:WB-Ce, Human, HepG2 cells.
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Lipid-Induced Toxicity Stimulates Hepatocytes to Release Angiogenic Microparticles That Require Vanin-1 for Uptake by Endothelial Cells.
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