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IARS 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003376-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-IARS full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 116.16
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-IARS antibody (H00003376-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00003376-T01
    IARS transfected lysate.
    Western Blot
    QC Testing of H00003376-T01
    Lane 1: IARS transfected lysate ( 116.16 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3376
  • Gene Name:
  • IARS
  • Gene Alias:
  • FLJ20736,IARS1,ILRS,PRO0785
  • Gene Description:
  • isoleucyl-tRNA synthetase
  • Gene Summary:
  • Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq
  • Other Designations:
  • isoleucine tRNA ligase 1, cytoplasmic,isoleucine-tRNA synthetase
  • Related Disease
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