IARS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-IARS full-length
Host
Human
Theoretical MW (kDa)
116.16
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-IARS antibody (H00003376-B01) by Western Blots.
SDS-PAGE Gel
IARS transfected lysate.
Western Blot
Lane 1: IARS transfected lysate ( 116.16 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — IARS
Entrez GeneID
3376GeneBank Accession#
BC065552.1Protein Accession#
AAH65552.1Gene Name
IARS
Gene Alias
FLJ20736, IARS1, ILRS, PRO0785
Gene Description
isoleucyl-tRNA synthetase
Omim ID
600709Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq
Other Designations
isoleucine tRNA ligase 1, cytoplasmic|isoleucine-tRNA synthetase
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Interactome
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Pathway
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Disease
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