IARS (Human) Recombinant Protein (Q01)
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More Files
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Specification
Product Description
Human IARS partial ORF ( NP_038203.1, 1172 a.a. - 1262 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
QYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
35.75
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — IARS
Entrez GeneID
3376GeneBank Accession#
NM_013417Protein Accession#
NP_038203.1Gene Name
IARS
Gene Alias
FLJ20736, IARS1, ILRS, PRO0785
Gene Description
isoleucyl-tRNA synthetase
Omim ID
600709Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq
Other Designations
isoleucine tRNA ligase 1, cytoplasmic|isoleucine-tRNA synthetase
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Interactome
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Pathway
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Disease
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