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HUS1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003364-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-HUS1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 30.91
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-HUS1 antibody (H00003364-B01) by Western Blots.
    Western Blot
    QC Testing of H00003364-T01
    Lane 1: HUS1 transfected lysate ( 31.7 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00003364-T01
    HUS1 transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3364
  • Gene Name:
  • HUS1
  • Gene Alias:
  • -
  • Gene Description:
  • HUS1 checkpoint homolog (S. pombe)
  • Gene Summary:
  • The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. [provided by RefSeq
  • Other Designations:
  • HUS1 checkpoint protein,hus1+-like protein
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