HSD3B2 monoclonal antibody (M02), clone 1E8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of HSD3B2 expression in transfected 293T cell line by HSD3B2 monoclonal antibody (M02), clone 1E8.
Lane 1: HSD3B2 transfected lysate(42.1 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HSD3B2 is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (35.64 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant HSD3B2.
Immunogen
HSD3B2 (NP_000189.1, 33 a.a. ~ 122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ALDKAFRPELREEFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (72)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.64 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of HSD3B2 expression in transfected 293T cell line by HSD3B2 monoclonal antibody (M02), clone 1E8.
Lane 1: HSD3B2 transfected lysate(42.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HSD3B2 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — HSD3B2
Entrez GeneID
3284GeneBank Accession#
NM_000198Protein Accession#
NP_000189.1Gene Name
HSD3B2
Gene Alias
HSDB, HSDB3, SDR11E2
Gene Description
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Omim ID
201810Gene Ontology
HyperlinkGene Summary
O
Other Designations
3 beta-HSD type II|3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II|OTTHUMP00000014489|delta 5-delta 4-isomerase type II|hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 2|short ch
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Interactome
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Pathway
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Disease
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Publication Reference
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Pomegranate extracts impact the androgen biosynthesis pathways in prostate cancer models in vitro and in vivo.
Ming DS, Pham S, Deb S, Chin MY, Kharmate G, Adomat H, Beheshti EH, Locke J, Guns ET.
The Journal of Steroid Biochemistry and Molecular Biology 2014 Sep; 143:19.
Application：WB-Ce, Human, LNCaP, 22RV1 cells.
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Pomegranate extracts impact the androgen biosynthesis pathways in prostate cancer models in vitro and in vivo.
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