HSD3B1 monoclonal antibody (M01J), clone 3C11-D4
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant HSD3B1.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
HSD3B1 (AAH31999.1, 1 a.a. ~ 373 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVLDKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLKRACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLKNGGTLYTCALRPMYIYGEGSRFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKKAPSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRLDSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPFNRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKENLKSKTQ
Host
Mouse
Reactivity
Human
Preparation Method
Cell Culture Production
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (66.77 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
HSD3B1 monoclonal antibody (M01J), clone 3C11-D4. Western Blot analysis of HSD3B1 expression in human placenta.Western Blot (Transfected lysate)
Western Blot analysis of HSD3B1 expression in transfected 293T cell line by HSD3B1 monoclonal antibody (M01J), clone 3C11-D4.
Lane 1: HSD3B1 transfected lysate (Predicted MW: 42.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to HSD3B1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HSD3B1 is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — HSD3B1
Entrez GeneID
3283GeneBank Accession#
BC031999Protein Accession#
AAH31999.1Gene Name
HSD3B1
Gene Alias
HSD3B, HSDB3, SDR11E1
Gene Description
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Omim ID
109715Gene Ontology
HyperlinkGene Summary
O
Other Designations
hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1|short chain dehydrogenase/reductase family 11E, member 1
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Interactome
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Pathway
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Disease
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