HSD3B1 monoclonal antibody (M01), clone 3C11-D4
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More Files
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Western Blot (Cell lysate)
HSD3B1 monoclonal antibody (M01), clone 3C11-D4. Western Blot analysis of HSD3B1 expression in human placenta.
Western Blot (Transfected lysate)
Western Blot analysis of HSD3B1 expression in transfected 293T cell line by HSD3B1 monoclonal antibody (M01), clone 3C11-D4.
Lane 1: HSD3B1 transfected lysate (Predicted MW: 42.3 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to HSD3B1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of various tissues with hematoxylin and HSD3B1 antibody under high magnification. [antibody concentration 3 ug/ml]
( a ) Stomach
( b ) Esophagus
( c ) Endometrium
( d ) Uterine cervix
( e ) Placenta
( f ) Ovary, clear cell carcinoma
( g ) Hepatocellular carcinoma
( h ) Breast cancer
( i ) Colon adenocarcinoma
( j and k ) Cervical carcinoma
( l, m, and n ) Choriocarcinoma
( o ) Epithelioid trophoblastic tumor
Western Blot detection against Immunogen (66.77 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant HSD3B1.
Immunogen
HSD3B1 (AAH31999.1, 1 a.a. ~ 373 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVLDKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLKRACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLKNGGTLYTCALRPMYIYGEGSRFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKKAPSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRLDSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPFNRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKENLKSKTQ
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (66.77 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HSD3B1 monoclonal antibody (M01), clone 3C11-D4. Western Blot analysis of HSD3B1 expression in human placenta.Western Blot (Transfected lysate)
Western Blot analysis of HSD3B1 expression in transfected 293T cell line by HSD3B1 monoclonal antibody (M01), clone 3C11-D4.
Lane 1: HSD3B1 transfected lysate (Predicted MW: 42.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to HSD3B1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of various tissues with hematoxylin and HSD3B1 antibody under high magnification. [antibody concentration 3 ug/ml]
( a ) Stomach
( b ) Esophagus
( c ) Endometrium
( d ) Uterine cervix
( e ) Placenta
( f ) Ovary, clear cell carcinoma
( g ) Hepatocellular carcinoma
( h ) Breast cancer
( i ) Colon adenocarcinoma
( j and k ) Cervical carcinoma
( l, m, and n ) Choriocarcinoma
( o ) Epithelioid trophoblastic tumorELISA
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Gene Info — HSD3B1
Entrez GeneID
3283GeneBank Accession#
BC031999Protein Accession#
AAH31999.1Gene Name
HSD3B1
Gene Alias
HSD3B, HSDB3, SDR11E1
Gene Description
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Omim ID
109715Gene Ontology
HyperlinkGene Summary
O
Other Designations
hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1|short chain dehydrogenase/reductase family 11E, member 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Intracrine activity involving NAD-dependent circadian steroidogenic activity governs age-associated meibomian gland dysfunction.
Lena Sasaki, Yuki Hamada, Daisuke Yarimizu, Tomo Suzuki, Hiroki Nakamura, Aya Shimada, Khanh Tien Nguyen Pham, Xinyan Shao, Koki Yamamura, Tsutomu Inatomi, Hironobu Morinaga, Emi K Nishimura, Fujimi Kudo, Ichiro Manabe, Shogo Haraguchi, Yuki Sugiura, Makoto Suematsu, Shigeru Kinoshita, Mamiko Machida, Takeshi Nakajima, Hiroshi Kiyonari, Hitoshi Okamura, Yoshiaki Yamaguchi, Takahito Miyake, Masao Doi.
Nature Aging 2022 Feb; 2(2):105.
Application：IHC, Human, Human eyelid.
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A case of primary aldosteronism caused by unilateral multiple adrenocortical micronodules presenting as muscle cramps at rest: The importance of functional histopathology for identifying a culprit lesion.
Ito A, Yamazaki Y, Sasano H, Matsubara D, Fukushima N, Tamba M, Tabata K, Ashizawa K, Takei A, Koizumi M, Sakuma Y, Sata N, Oshiro H.
Pathology International 2017 Apr; 67(4):214.
Application：IHC, Human, Human adrenocortical macronodules.
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Expression of steroidogenic enzymes and their transcription factors in cortisol-producing adrenocortical adenomas: immunohistochemical analysis and quantitative RT-PCR studies.
Kubota F, Nakamura Y, Konosu-Fukaya S, Azmahani A, Ise K, Yamazaki Y, Kitawaki Y, Felizola SJ, Ono Y, Omata K, Morimoto R, Iwama N, Satoh F, Sasano H.
Human pathology 2016 Aug; 54:165.
Application：IHC, Human, Human adrenal glands.
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Steroidogenic enzymes, their related transcription factors and nuclear receptors in human sebaceous glands under normal and pathological conditions.
Azmahani A, Nakamura Y, Felizola SJ, Ozawa Y, Ise K, Inoue T, McNamara KM, Doi M, Okamura H, Zouboulis CC, Aiba S, Sasano H.
The Journal of Steroid Biochemistry and Molecular Biology 2014 Oct; 144 Pt B:268.
Application：IHC, Human, Skin.
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Advances in the diagnosis of gestational trophoblastic tumors and tumor-like lesions.
Mao TL, Shih IM.
Expert Opinion on Medical Diagnostics 2009 Jul; 3(4):371.
Application：IHC, Human, Placental site trophoblastic tumor.
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Chorangiocarcinoma: A Case Report and Review of the Literature.
Ariel I, Boldes R, Weintraub A, Reinus C, Beller U, Arbel R.
International Journal of Gynecological Pathology 2009 May; 28(3):267.
Application：IHC, Human, Placenta.
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HSD3B1 as a novel trophoblast-associated marker that assists in the differential diagnosis of trophoblastic tumors and tumorlike lesions.
Mao TL, Kurman RJ, Jeng YM, Huang W, Shih IM.
The American Journal of Surgical Pathology 2008 Feb; 32(2):236.
Application：IHC, WB-Ti, Human, Normal and tumor tissues, including kidney, liver, colon, stomach, spleen, thyroid, pancreas, placenta and carcinomas from ovary, lung, and tongue.
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Trophogram, an immunohistochemistry-based algorithmic approach, in the differential diagnosis of trophoblastic tumors and tumorlike lesions.
Shih IeM.
Annals of Diagnostic Pathology 2007 Jun; 11(3):228.
Application：IHC, Human, Human trophoblastic tumors.
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Intracrine activity involving NAD-dependent circadian steroidogenic activity governs age-associated meibomian gland dysfunction.
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