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Last updated: 2016/12/4
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HSD3B1 monoclonal antibody (M01), clone 3C11-D4

  • Catalog # : H00003283-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full length recombinant HSD3B1.
  • Immunogen:
  • HSD3B1 (AAH31999.1, 1 a.a. ~ 373 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVLDKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLKRACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLKNGGTLYTCALRPMYIYGEGSRFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKKAPSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRLDSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPFNRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKENLKSKTQ
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG1 Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00003283-M01
    Western Blot detection against Immunogen (66.77 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • HSD3B1 monoclonal antibody (M01), clone 3C11-D4. Western Blot analysis of HSD3B1 expression in human placenta.
  • PDF DownloadProtocol Download
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of HSD3B1 expression in transfected 293T cell line by HSD3B1 monoclonal antibody (M01), clone 3C11-D4.

    Lane 1: HSD3B1 transfected lysate (Predicted MW: 42.3 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) enlargeenlarge this image
  • Immunoperoxidase of monoclonal antibody to HSD3B1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
  • PDF DownloadProtocol Download
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) enlargeenlarge this image
  • Immunohistochemical staining of various tissues with hematoxylin and HSD3B1 antibody under high magnification. [antibody concentration 3 ug/ml]
    ( a ) Stomach
    ( b ) Esophagus
    ( c ) Endometrium
    ( d ) Uterine cervix
    ( e ) Placenta
    ( f ) Ovary, clear cell carcinoma
    ( g ) Hepatocellular carcinoma
    ( h ) Breast cancer
    ( i ) Colon adenocarcinoma
    ( j and k ) Cervical carcinoma
    ( l, m, and n ) Choriocarcinoma
    ( o ) Epithelioid trophoblastic tumor
  • PDF DownloadProtocol Download
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3283
  • Gene Name:
  • HSD3B1
  • Gene Alias:
  • HSD3B,HSDB3,SDR11E1
  • Gene Description:
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
  • Other Designations:
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1,short chain dehydrogenase/reductase family 11E, member 1
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