HRG 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: HRG transfected lysate ( 59.6 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
HRG transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HRG full-length
Host
Human
Theoretical MW (kDa)
57.86
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HRG antibody (H00003273-B01) by Western Blots.
Western Blot
Lane 1: HRG transfected lysate ( 59.6 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
HRG transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HRG
Entrez GeneID
3273GeneBank Accession#
NM_000412Protein Accession#
NP_000403Gene Name
HRG
Gene Alias
DKFZp779H1622, HPRG, HRGP
Gene Description
histidine-rich glycoprotein
Omim ID
142640Gene Ontology
HyperlinkGene Summary
This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. [provided by RefSeq
Other Designations
histidine-proline rich glycoprotein|thrombophilia due to elevated HRG
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Interactome
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Disease
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