HPX monoclonal antibody (M01), clone 3A9-1A9
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant HPX.
Immunogen
HPX (AAH05395, 1 a.a. ~ 254 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MARVLGAPVALGLWSLCWSLAIATPLPPTSAHGNVAEGETKADPDVTERCSDGWSFDATTLDDNGTMLFFKGEFVWKSHKWDRELISERWKNFPSPVDAAFRQGHNSVFLIKGDKVWVYPPEKKEKGYPKLLQDEFPGIPSPLDAAVECHRGECQAEGVLFFQGDREWFWDLATGTMKERSWPAVGNCSSALRWLGRYYCFQGNQFLRFDPVRGEVPPRYPRDVRDYFMPCPGRGHGHRNGTGHGNSICAVAHI
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (53.68 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
HPX monoclonal antibody (M01), clone 3A9-1A9. Western Blot analysis of HPX expression in human liver.Western Blot (Transfected lysate)
Western Blot analysis of HPX expression in transfected 293T cell line by HPX monoclonal antibody (M01), clone 3A9-1A9.
Lane 1: HPX transfected lysate (Predicted MW: 28.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of HPX transfected lysate using anti-HPX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HPX MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HPX is 0.3 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of HPX over-expressed 293 cell line, cotransfected with HPX Validated Chimera RNAi ( Cat # H00003263-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HPX monoclonal antibody (M01), clone 3A9-1A9 (Cat # H00003263-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — HPX
Entrez GeneID
3263GeneBank Accession#
BC005395Protein Accession#
AAH05395Gene Name
HPX
Gene Alias
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Gene Description
hemopexin
Omim ID
142290Gene Ontology
HyperlinkGene Summary
This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq
Other Designations
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Interactome
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Disease
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