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Last updated: 2016/12/4
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HPX monoclonal antibody (M01), clone 3A9-1A9

  • Catalog # : H00003263-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full length recombinant HPX.
  • Immunogen:
  • HPX (AAH05395, 1 a.a. ~ 254 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • MARVLGAPVALGLWSLCWSLAIATPLPPTSAHGNVAEGETKADPDVTERCSDGWSFDATTLDDNGTMLFFKGEFVWKSHKWDRELISERWKNFPSPVDAAFRQGHNSVFLIKGDKVWVYPPEKKEKGYPKLLQDEFPGIPSPLDAAVECHRGECQAEGVLFFQGDREWFWDLATGTMKERSWPAVGNCSSALRWLGRYYCFQGNQFLRFDPVRGEVPPRYPRDVRDYFMPCPGRGHGHRNGTGHGNSICAVAHI
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2b Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00003263-M01
    Western Blot detection against Immunogen (53.68 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • HPX monoclonal antibody (M01), clone 3A9-1A9. Western Blot analysis of HPX expression in human liver.
  • PDF DownloadProtocol Download
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of HPX expression in transfected 293T cell line by HPX monoclonal antibody (M01), clone 3A9-1A9.

    Lane 1: HPX transfected lysate (Predicted MW: 28.6 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation of HPX transfected lysate using anti-HPX monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HPX MaxPab rabbit polyclonal antibody.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged HPX is 0.3 ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • Western blot analysis of HPX over-expressed 293 cell line, cotransfected with HPX Validated Chimera RNAi ( Cat # H00003263-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HPX monoclonal antibody (M01), clone 3A9-1A9 (Cat # H00003263-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3263
  • Gene Name:
  • HPX
  • Gene Alias:
  • -
  • Gene Description:
  • hemopexin
  • Gene Summary:
  • This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq
  • Other Designations:
  • -
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