HPX monoclonal antibody (M01), clone 3A9-1A9

Catalog # H00003263-M01

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Size:100 ug
Price: USD $ 335.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Tissue lysate)
Application

Western Blot (Tissue lysate)

HPX monoclonal antibody (M01), clone 3A9-1A9. Western Blot analysis of HPX expression in human liver.

Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western Blot analysis of HPX expression in transfected 293T cell line by HPX monoclonal antibody (M01), clone 3A9-1A9.

Lane 1: HPX transfected lysate (Predicted MW: 28.6 KDa).
Lane 2: Non-transfected lysate.

Immunoprecipitation
Application

Immunoprecipitation

Immunoprecipitation of HPX transfected lysate using anti-HPX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HPX MaxPab rabbit polyclonal antibody.

Sandwich ELISA (Recombinant protein)
Application

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged HPX is 0.3 ng/ml as a capture antibody.

RNAi Knockdown (Antibody validated)
Application

RNAi Knockdown (Antibody validated)

Western blot analysis of HPX over-expressed 293 cell line, cotransfected with HPX Validated Chimera RNAi ( Cat # H00003263-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HPX monoclonal antibody (M01), clone 3A9-1A9 (Cat # H00003263-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

QC Test

Western Blot detection against Immunogen (53.68 KDa) .

  • Specification

    Product Description

    Mouse monoclonal antibody raised against a full length recombinant HPX.

    Immunogen

    HPX (AAH05395, 1 a.a. ~ 254 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Sequence

    MARVLGAPVALGLWSLCWSLAIATPLPPTSAHGNVAEGETKADPDVTERCSDGWSFDATTLDDNGTMLFFKGEFVWKSHKWDRELISERWKNFPSPVDAAFRQGHNSVFLIKGDKVWVYPPEKKEKGYPKLLQDEFPGIPSPLDAAVECHRGECQAEGVLFFQGDREWFWDLATGTMKERSWPAVGNCSSALRWLGRYYCFQGNQFLRFDPVRGEVPPRYPRDVRDYFMPCPGRGHGHRNGTGHGNSICAVAHI

    Host

    Mouse

    Reactivity

    Human

    Isotype

    IgG2b Kappa

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Western Blot detection against Immunogen (53.68 KDa) .

    Storage Buffer

    In 1x PBS, pH 7.4

    Storage Instruction

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot (Tissue lysate)

    HPX monoclonal antibody (M01), clone 3A9-1A9. Western Blot analysis of HPX expression in human liver.

    Western Blot (Transfected lysate)

    Western Blot analysis of HPX expression in transfected 293T cell line by HPX monoclonal antibody (M01), clone 3A9-1A9.

    Lane 1: HPX transfected lysate (Predicted MW: 28.6 KDa).
    Lane 2: Non-transfected lysate.

    Western Blot (Recombinant protein)

    Immunoprecipitation

    Immunoprecipitation of HPX transfected lysate using anti-HPX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HPX MaxPab rabbit polyclonal antibody.

    Sandwich ELISA (Recombinant protein)

    Detection limit for recombinant GST tagged HPX is 0.3 ng/ml as a capture antibody.

    ELISA

    RNAi Knockdown (Antibody validated)

    Western blot analysis of HPX over-expressed 293 cell line, cotransfected with HPX Validated Chimera RNAi ( Cat # H00003263-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HPX monoclonal antibody (M01), clone 3A9-1A9 (Cat # H00003263-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
  • Gene Info — HPX

    Entrez GeneID

    3263

    GeneBank Accession#

    BC005395

    Protein Accession#

    AAH05395

    Gene Name

    HPX

    Gene Alias

    -

    Gene Description

    hemopexin

    Omim ID

    142290

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq

    Other Designations

    -

  • Interactome
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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