HPRT1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HPRT1 full-length
Host
Human
Theoretical MW (kDa)
24.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HPRT1 antibody (H00003251-D01F) by Western Blots.
SDS-PAGE Gel
HPRT1 transfected lysate.
Western Blot
Lane 1: HPRT1 transfected lysate ( 24.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HPRT1
Entrez GeneID
3251GeneBank Accession#
NM_000194.1Protein Accession#
NP_000185.1Gene Name
HPRT1
Gene Alias
HGPRT, HPRT
Gene Description
hypoxanthine phosphoribosyltransferase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout
Other Designations
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Interactome
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