HPRT1 monoclonal antibody (M01), clone 4C3-G8
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant HPRT1.
Immunogen
HPRT1 (AAH00578, 1 a.a. ~ 218 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MATRSPGVVISDDEPGYDLDLFCIPNHYAEDLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA
Host
Mouse
Reactivity
Human
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (49.72 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HPRT1 monoclonal antibody (M01), clone 4C3-G8 Western Blot analysis of HPRT1 expression in MCF-7 ( Cat # L046V1 ).Western Blot (Recombinant protein)
ELISA
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Gene Info — HPRT1
Entrez GeneID
3251GeneBank Accession#
BC000578Protein Accession#
AAH00578Gene Name
HPRT1
Gene Alias
HGPRT, HPRT
Gene Description
hypoxanthine phosphoribosyltransferase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Sorting nexin 27 interacts with multidrug resistance-associated protein 4 (MRP4) and mediates internalization of MRP4.
Hayashi H, Naoi S, Nakagawa T, Nishikawa T, Fukuda H, Imajoh-Ohmi S, Kondo A, Kubo K, Yabuki T, Hattori A, Hirouchi M, Sugiyama Y.
The Journal of Biological Chemistry 2012 Apr; 287(18):15054.
Application:WB-Tr, Human, HEK 293 cells.
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Sorting nexin 27 interacts with multidrug resistance-associated protein 4 (MRP4) and mediates internalization of MRP4.
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