Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi ( Cat # H00003172-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HNF4A monoclonal antibody (M04), clone 4E2 (Cat # H00003172-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq