HMOX2 monoclonal antibody (M01), clone 1D8-1A8
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant HMOX2.
Immunogen
HMOX2 (AAH02396, 1 a.a. ~ 316 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSAEVETSEGVDESEKKNSGALEKENQMRMADLSELLKEGTKEAHDRAENTQFVKDFLKGNIKKELFKLATTALYFTYSALEEEMERNKDHPAFAPLYFPMELHRKEALTKDMEYFFGENWEEQVQCPKAAQKYVERIHYIGQNEPELLVAHAYTRYMGDLSGGQVLKKVAQRALKLPSTGEGTQFYLFENVDNAQQFKQLYRARMNALDLNMKTKERIVEEANKAFEYNMQIFNELDQAGSTLARETLEDGFPVHDGKGDMRKCPFYAAEQDKGALEGSSCPFRTAMAVLRKPSLQFILAAGVALAAGLLAWYYM
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (89); Rat (90)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (60.5 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
HMOX2 monoclonal antibody (M01), clone 1D8-1A8. Western Blot analysis of HMOX2 expression in human spleen.Western Blot (Cell lysate)
HMOX2 monoclonal antibody (M01), clone 1D8-1A8 Western Blot analysis of HMOX2 expression in HepG2 ( Cat # L019V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HMOX2 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to HMOX2 on HeLa cell. [antibody concentration1 ~ 10 ug/ml] -
Gene Info — HMOX2
Entrez GeneID
3163GeneBank Accession#
BC002396Protein Accession#
AAH02396Gene Name
HMOX2
Gene Alias
HO-2
Gene Description
heme oxygenase (decycling) 2
Omim ID
141251Gene Ontology
HyperlinkGene Summary
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus. [provided by RefSeq
Other Designations
heme oxygenase (decyclizing) 2
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Interactome
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Pathway
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Disease
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Publication Reference
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An enzyme that selectively S-nitrosylates proteins to regulate insulin signaling.
Hua-Lin Zhou, Zachary W Grimmett, Nicholas M Venetos, Colin T Stomberski, Zhaoxia Qian, Precious J McLaughlin, Puneet K Bansal, Rongli Zhang, James D Reynolds, Richard T Premont, Jonathan S Stamler.
Cell 2023 Dec; 186(26):5812.
Application:WB, Human, HEK293T cells.
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Anti-apoptotic properties of carbon monoxide in porcine oocyte during in vitro aging.
Němeček D, Dvořáková M, Heroutová I, Chmelíková E, Sedmíková M.
PeerJ 2017 Oct; 5:e3876.
Application:ICC, IF, Pig, Pig oocytes.
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An enzyme that selectively S-nitrosylates proteins to regulate insulin signaling.
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