HMGCS2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HMGCS2 full-length
Host
Human
Theoretical MW (kDa)
55.99
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HMGCS2 antibody (H00003158-B01) by Western Blots.
SDS-PAGE Gel
HMGCS2 transfected lysate.
Western Blot
Lane 1: HMGCS2 transfected lysate ( 55.99 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HMGCS2
Entrez GeneID
3158GeneBank Accession#
NM_005518.2Protein Accession#
NP_005509.1Gene Name
HMGCS2
Gene Alias
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Gene Description
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial)
Gene Ontology
HyperlinkGene Summary
Mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase (HMGCS2; EC 2.3.3.10) mediates the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for brain, heart, kidney, and other organs during times of carbohydrate deprivation such as fasting (Robinson and Williamson, 1980 [PubMed 6986618]). Also see cytoplasmic HMG-CoA synthase (HMGCS1; MIM 142940), which mediates an early step in cholesterol synthesis.[supplied by OMIM
Other Designations
OTTHUMP00000013928|hydroxymethylglutaryl-CoA synthase 2
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