HMGCS2 monoclonal antibody (M06), clone 1E9
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant HMGCS2.
Immunogen
HMGCS2 (NP_005509.1, 424 a.a. ~ 508 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
RVSQDAAPGSPLDKLVSSTSDLPKRLASRKCVSPEEFTEIMNQREQFYHKVNFSPPGDTNSLFPGTWYLERVDEQHRRKYARRPV
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.09 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Transfected lysate)
Western Blot analysis of HMGCS2 expression in transfected 293T cell line by HMGCS2 monoclonal antibody (M06), clone 1E9.
Lane 1: HMGCS2 transfected lysate(56.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HMGCS2 is approximately 1ng/ml as a capture antibody.ELISA
-
Gene Info — HMGCS2
Entrez GeneID
3158GeneBank Accession#
NM_005518Protein Accession#
NP_005509.1Gene Name
HMGCS2
Gene Alias
-
Gene Description
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial)
Gene Ontology
HyperlinkGene Summary
Mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase (HMGCS2; EC 2.3.3.10) mediates the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for brain, heart, kidney, and other organs during times of carbohydrate deprivation such as fasting (Robinson and Williamson, 1980 [PubMed 6986618]). Also see cytoplasmic HMG-CoA synthase (HMGCS1; MIM 142940), which mediates an early step in cholesterol synthesis.[supplied by OMIM
Other Designations
OTTHUMP00000013928|hydroxymethylglutaryl-CoA synthase 2
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Human Mitochondrial HMG-CoA Synthase Deficiency: Role of Enzyme Dimerization Surface and Characterization of Three New Patients.
Puisac B, Marcos-Alcalde I, Hernández-Marcos M, Tobajas Morlana P, Levtova A, Schwahn BC, DeLaet C, Lace B, Gómez-Puertas P, Pié J.
International Journal of Molecular Sciences 2018 Mar; 19(4):E1010.
Application:WB-Re, Human, Recombinant protein.
-
Human Mitochondrial HMG-CoA Synthase Deficiency: Role of Enzyme Dimerization Surface and Characterization of Three New Patients.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com