Immunoprecipitation of HMGCR transfected lysate using anti-HMGCR MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HMGCR purified MaxPab mouse polyclonal antibody (B01P) (H00003156-B01P).
HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
3-hydroxy-3-methylglutaryl CoA reductase (NADPH),hydroxymethylglutaryl-CoA reductase