HMBS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
HMBS transfected lysate.
Western Blot
Lane 1: HMBS transfected lysate ( 39.82 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HMBS full-length
Host
Human
Theoretical MW (kDa)
39.82
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HMBS antibody (H00003145-B01) by Western Blots.
SDS-PAGE Gel
HMBS transfected lysate.
Western Blot
Lane 1: HMBS transfected lysate ( 39.82 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HMBS
Entrez GeneID
3145GeneBank Accession#
NM_000190.3Protein Accession#
-Gene Name
HMBS
Gene Alias
PBG-D, PBGD, UPS
Gene Description
hydroxymethylbilane synthase
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
porphobilinogen deaminase|pre-uroporphyrinogen synthase|uroporphyrinogen I synthase|uroporphyrinogen I synthetase
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Interactome
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Pathway
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Disease
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