HLCS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
HLCS transfected lysate.
Western Blot
Lane 1: HLCS transfected lysate ( 79.97 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HLCS full-length
Host
Human
Theoretical MW (kDa)
79.97
Interspecies Antigen Sequence
Mouse (77)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HLCS antibody (H00003141-B01) by Western Blots.
SDS-PAGE Gel
HLCS transfected lysate.
Western Blot
Lane 1: HLCS transfected lysate ( 79.97 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HLCS
Entrez GeneID
3141GeneBank Accession#
NM_000411.4Protein Accession#
NP_000402.3Gene Name
HLCS
Gene Alias
HCS
Gene Description
holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
Gene Ontology
HyperlinkGene Summary
Holocarboxylase synthetase (EC 6.3.4.10) covalently links biotin to propionyl-CoA carboxylase (PCCA; MIM 232000), pyruvate carboxylase (PC; MIM 608786), alpha-methylcrotonyl-CoA carboxylase (MCCC1; MIM 609010), and acetyl-CoA carboxylase (ACACA; MIM 200350).[supplied by OMIM
Other Designations
OTTHUMP00000109039|biotin apo-protein ligase|holocarboxylase synthetase
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Interactome
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Pathway
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