HLA-E 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HLA-E full-length
Host
Human
Theoretical MW (kDa)
40.2
Interspecies Antigen Sequence
Mouse (69); Rat (62)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HLA-E antibody (H00003133-B01) by Western Blots.
SDS-PAGE Gel
HLA-E transfected lysate
Western Blot
Lane 1: HLA-E transfected lysate ( 40.2 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HLA-E
Entrez GeneID
3133GeneBank Accession#
BC002578Protein Accession#
AAH02578Gene Name
HLA-E
Gene Alias
DKFZp686P19218, EA1.2, EA2.1, HLA-6.2, MHC, QA1
Gene Description
major histocompatibility complex, class I, E
Omim ID
143010Gene Ontology
HyperlinkGene Summary
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq
Other Designations
HLA class I histocompatibility antigen, E alpha chain|MHC HLA-E alpha-1|MHC HLA-E alpha-2.1|MHC class I antigen|OTTHUMP00000029214|lymphocyte antigen
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Interactome
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Pathway
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Disease
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