HLA-E purified MaxPab mouse polyclonal antibody (B01P)
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human HLA-E protein.
Immunogen
HLA-E (AAH02578.1, 1 a.a. ~ 358 a.a) full-length human protein.
Sequence
MVDGTLLLLLSEALALTQTWAGSHSLKYFHTSVSRPGRGEPRFISVGYVDDTQFVRFDNDAASPRMVPRAPWMEQEGSEYWDRETRSARDTAQIFRVNLRTLRGYYNQSEAGSHTLQWMHGCELGPDRRFLRGYEQFAYDGKDYLTLNEDLRSWTAVDTAAQISEQKSNDASEAEHQRAYLEDTCVEWLHKYLEKGKETLLHLEPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQQDGEGHTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPVTLRWKPASQPTIPIVGIIAGLVLLGSVVSGAVVAAVIWRKKSSGGKGGSYSKAEWSDSAQGSESHSL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (69); Rat (62)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Flow Cytometry
FACS analysis of negative control 293 cells (Black) and HLA-E expressing 293 cells (Green) using HLA-E purified MaxPab mouse polyclonal antibody.Western Blot (Tissue lysate)
HLA-E MaxPab polyclonal antibody. Western Blot analysis of HLA-E expression in human spleen.Western Blot (Transfected lysate)
Western Blot analysis of HLA-E expression in transfected 293T cell line (H00003133-T01) by HLA-E MaxPab polyclonal antibody.
Lane 1: HLA-E transfected lysate(39.38 KDa).
Lane 2: Non-transfected lysate.
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Gene Info — HLA-E
Entrez GeneID
3133GeneBank Accession#
BC002578Protein Accession#
AAH02578.1Gene Name
HLA-E
Gene Alias
DKFZp686P19218, EA1.2, EA2.1, HLA-6.2, MHC, QA1
Gene Description
major histocompatibility complex, class I, E
Omim ID
143010Gene Ontology
HyperlinkGene Summary
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq
Other Designations
HLA class I histocompatibility antigen, E alpha chain|MHC HLA-E alpha-1|MHC HLA-E alpha-2.1|MHC class I antigen|OTTHUMP00000029214|lymphocyte antigen
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Interactome
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Pathway
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Disease
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