Western blot analysis of HLF over-expressed 293 cell line, cotransfected with HLF Validated Chimera RNAi ( Cat # H00003131-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HLF monoclonal antibody (M04), clone M2 (Cat # H00003131-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq