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HLA-DRB3 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003125-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-HLA-DRB3 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 30
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-HLA-DRB3 antibody (H00003125-B02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00003125-T01
    HLA-DRB3 transfected lysate
    Western Blot
    QC Testing of H00003125-T01
    Lane 1: HLA-DRB3 transfected lysate ( 30 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3125
  • Gene Name:
  • HLA-DRB3
  • Gene Alias:
  • HLA-DR3B,MGC117330
  • Gene Description:
  • major histocompatibility complex, class II, DR beta 3
  • Gene Summary:
  • HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB3 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq
  • Other Designations:
  • MHC class II HLA-DR beta 3 chain,MHC class II antigen DR beta 3 chain,human leucocyte antigen DRB3
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