HLA-DPA1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HLA-DPA1 full-length
Host
Human
Theoretical MW (kDa)
29.3
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HLA-DPA1 antibody (H00003113-D01P) by Western Blots.
SDS-PAGE Gel
HLA-DPA1 transfected lysate.
Western Blot
Lane 1: HLA-DPA1 transfected lysate ( 29.30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HLA-DPA1
Entrez GeneID
3113GeneBank Accession#
BC009956Protein Accession#
AAH09956.1Gene Name
HLA-DPA1
Gene Alias
HLA-DP1A, HLADP, HLASB
Gene Description
major histocompatibility complex, class II, DP alpha 1
Omim ID
142880Gene Ontology
HyperlinkGene Summary
HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq
Other Designations
HLA class II histocompatibility antigen, DP alpha chain|MHC class II HLA-DPA1 antigen|MHC class II antigen|OTTHUMP00000029066|OTTHUMP00000062983
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Interactome
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