HLA-DPA1 monoclonal antibody (M03), clone 1E3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3.
Lane 1: HLA-DPA1 transfected lysate(29.3 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3ng/ml as a capture antibody.
Western Blot detection against Immunogen (54.34 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant HLA-DPA1.
Immunogen
HLA-DPA1 (AAH09956, 1 a.a. ~ 260 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MRPEDRMFHIRAVILRALSLAFLLSLRGAGAIKADHVSTYAAFVQTHRPTGEFMFEFDEDEQFYVDLDKKETVWHLEEFGRAFSFEAQGGLANIAILNNNLNTLIQRSNHTQAANDPPEVTVFPKEPVELGQPNTLICHIDRFFPPVLNVTWLCNGEPVTEGVAESLFLPRTDYSFHKFHYLTFVPSAEDVYDCRVEHWGLDQPLLKHWEAQEPIQMPETTETVLCALGLVLGLVGIIVGTVLIIKSLRSGHDPRAQGPL
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (54.34 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3.
Lane 1: HLA-DPA1 transfected lysate(29.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3ng/ml as a capture antibody.ELISA
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Gene Info — HLA-DPA1
Entrez GeneID
3113GeneBank Accession#
BC009956Protein Accession#
AAH09956Gene Name
HLA-DPA1
Gene Alias
HLA-DP1A, HLADP, HLASB
Gene Description
major histocompatibility complex, class II, DP alpha 1
Omim ID
142880Gene Ontology
HyperlinkGene Summary
HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq
Other Designations
HLA class II histocompatibility antigen, DP alpha chain|MHC class II HLA-DPA1 antigen|MHC class II antigen|OTTHUMP00000029066|OTTHUMP00000062983
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Interactome
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Pathway
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Disease
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