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HLA-DOB 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003112-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-HLA-DOB full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 30.8
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-HLA-DOB antibody (H00003112-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00003112-T01
    HLA-DOB transfected lysate
    Western Blot
    QC Testing of H00003112-T01
    Lane 1: HLA-DOB transfected lysate ( 30.8 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3112
  • Gene Name:
  • HLA-DOB
  • Gene Alias:
  • DOB
  • Gene Description:
  • major histocompatibility complex, class II, DO beta
  • Gene Summary:
  • HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq
  • Other Designations:
  • HLA class II histocompatibility antigen, DO beta chain,OTTHUMP00000029312,class II beta chain
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