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HLA-A 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003105-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-HLA-A full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 40.8
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-HLA-A antibody (H00003105-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00003105-T02
    HLA-A transfected lysate.
    Western Blot
    QC Testing of H00003105-T02
    Lane 1: HLA-A transfected lysate ( 40.80 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3105
  • Gene Name:
  • HLA-A
  • Gene Alias:
  • HLAA
  • Gene Description:
  • major histocompatibility complex, class I, A
  • Gene Summary:
  • HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq
  • Other Designations:
  • HLA class I histocompatibility antigen, A-23 alpha chain,MHC class I antigen HLA-A heavy chain,MHC leukocyte antigen,OTTHUMP00000161059,antigen presenting molecule,leucocyte antigen class I,leukocyte antigen class I-A
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