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HIF1A 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003091-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-HIF1A full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 82.7
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-HIF1A antibody (H00003091-B01) by Western Blots.
    Western Blot
    QC Testing of H00003091-T01
    Lane 1: HIF1A transfected lysate ( 82.7 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00003091-T01
    HIF1A transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3091
  • Gene Name:
  • HIF1A
  • Gene Alias:
  • HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78
  • Gene Description:
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • Gene Summary:
  • Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • ARNT interacting protein,hypoxia-inducible factor 1, alpha subunit,hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor),member of PAS superfamily 1
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