HIF1A 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HIF1A full-length
Host
Human
Theoretical MW (kDa)
82.7
Interspecies Antigen Sequence
Mouse (87); Rat (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HIF1A antibody (H00003091-B01) by Western Blots.
Western Blot
Lane 1: HIF1A transfected lysate ( 82.7 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
HIF1A transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HIF1A
Entrez GeneID
3091GeneBank Accession#
NM_181054Protein Accession#
NP_851397Gene Name
HIF1A
Gene Alias
HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Gene Description
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Omim ID
603348Gene Ontology
HyperlinkGene Summary
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
ARNT interacting protein|hypoxia-inducible factor 1, alpha subunit|hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|member of PAS superfamily 1
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