HIF1A (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human HIF1A full-length ORF ( NP_851397.1, 1 a.a. - 735 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGII
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
109.1
Interspecies Antigen Sequence
Mouse (87); Rat (89)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — HIF1A
Entrez GeneID
3091GeneBank Accession#
NM_181054.1Protein Accession#
NP_851397.1Gene Name
HIF1A
Gene Alias
HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Gene Description
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Omim ID
603348Gene Ontology
HyperlinkGene Summary
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
ARNT interacting protein|hypoxia-inducible factor 1, alpha subunit|hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|member of PAS superfamily 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Hypoxia-inducible factor-1α regulates PI3K/AKT signaling through microRNA-32-5p/PTEN and affects nucleus pulposus cell proliferation and apoptosis.
Daolu Zhan, Mingxia Lin, Jian Chen, Wentao Cai, Jian Liu, Yehan Fang, Yibo Li, Bin Wu, Guangji Wang.
Mediators of Inflammation 2021 Apr; 2021:1610251.
Application:Func, Human, Human nucleus pulposus cells.
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The E3 ubiquitin ligase Pellino3 protects against obesity-induced inflammation and insulin resistance.
Yang S, Wang B, Humphries F, Hogan AE, O’Shea D, Moynagh PN.
Immunity 2014 Dec; 41(6):973.
Application:WB-Ce, WB-Ti, WB-Tr, Human, Mouse, BMDMs, Liver, Epididymal fat, MEFs, HEK293T cells.
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A new method to assay hypoxia-inducible factor-1 based on small molecule binding DNA.
Yang N, Li H, Yin T, Huang Y, Sun L, Li G.
Analytica Chimica Acta 2014 Aug; 838:31.
Application:Func, Human, Placenta.
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Hypoxia Inducible Factors Promotes Alveolar Development and Regeneration.
Vadivel A, Alphonse RS, Etches N, van Haaften T, Collins JJ, O'Reilly M, Eaton F, Thébaud B.
American Journal of Respiratory Cell and Molecular Biology 2014 Jan; 50(1):96.
Application:WB-Ti, Rat, Lung.
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Hypoxia-inducible factor-1α regulates PI3K/AKT signaling through microRNA-32-5p/PTEN and affects nucleus pulposus cell proliferation and apoptosis.
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