HIC1 monoclonal antibody (M01), clone 4E11
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant HIC1.
Immunogen
HIC1 (NP_006488, 396 a.a. ~ 453 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
YKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLN
Host
Mouse
Reactivity
Human, Rat
Interspecies Antigen Sequence
Mouse (100)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (32.38 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HIC1 monoclonal antibody (M01), clone 4E11 Western Blot analysis of HIC1 expression in Jurkat ( Cat # L017V1 ).Western Blot (Cell lysate)
HIC1 monoclonal antibody (M01), clone 4E11. Western Blot analysis of HIC1 expression in PC-12(Cat # L012V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HIC1 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to HIC1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — HIC1
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Interactome
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Disease
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Publication Reference
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HIC1 and RassF1A Methylation Attenuates Tubulin Expression and Cell Stiffness in Cancer.
Chen CC, He BC, Chen YL, Lee KD, Tung CH, Hsu CC, Lin PY, Chu PY, Leu YW, Fu WE, Hsiao SH.
International Journal of Molecular Sciences 2018 Sep; 19(10):E2844.
Application:IF, WB, Mouse, Mouse mesenchymal stem cells.
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HIC1 and RassF1A Methylation Attenuates Tubulin Expression and Cell Stiffness in Cancer.
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