HGD 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
HGD transfected lysate.
Western Blot
Lane 1: HGD transfected lysate ( 49.06 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HGD full-length
Host
Human
Theoretical MW (kDa)
49.06
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HGD antibody (H00003081-B01) by Western Blots.
SDS-PAGE Gel
HGD transfected lysate.
Western Blot
Lane 1: HGD transfected lysate ( 49.06 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HGD
Entrez GeneID
3081GeneBank Accession#
NM_000187.1Protein Accession#
AAH71757.1Gene Name
HGD
Gene Alias
AKU, HGO
Gene Description
homogentisate 1,2-dioxygenase (homogentisate oxidase)
Gene Ontology
HyperlinkGene Summary
Homogentisate 1,2-dioxygenase (HGD) gene mutations are the molecular cause of alkaptonuria, a rare hereditary disorder of the phenylalanine catabolism. The highest expression of HGD is in the prostate, small intestine, colon, and liver. The HGD gene contains 14 exons. Conflicting reports have placed the gene at 3q2, 3q13.3-q21, 3q21-q24, 3q21-q23, or 3q25-q26. [provided by RefSeq
Other Designations
homogentisate 1,2-dioxygenase|homogentisicase
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