HFE 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HFE full-length
Host
Human
Theoretical MW (kDa)
40.1
Interspecies Antigen Sequence
Mouse (69); Rat (70)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HFE antibody (H00003077-B01) by Western Blots.
SDS-PAGE Gel
HFE transfected lysate.
Western Blot
Lane 1: HFE transfected lysate ( 40.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HFE
Entrez GeneID
3077GeneBank Accession#
NM_000410.3Protein Accession#
-Gene Name
HFE
Gene Alias
HFE1, HH, HLA-H, MGC103790, dJ221C16.10.1
Gene Description
hemochromatosis
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. [provided by RefSeq
Other Designations
MHC class I-like protein HFE|hemochromatosis protein|hereditary hemochromatosis protein HLA-H
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Interactome
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Disease
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