HEXA 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
HEXA transfected lysate.
Western Blot
Lane 1: HEXA transfected lysate ( 58.3 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HEXA full-length
Host
Human
Theoretical MW (kDa)
58.3
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HEXA antibody (H00003073-B01) by Western Blots.
SDS-PAGE Gel
HEXA transfected lysate.
Western Blot
Lane 1: HEXA transfected lysate ( 58.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HEXA
Entrez GeneID
3073GeneBank Accession#
NM_000520.2Protein Accession#
NP_000511.1Gene Name
HEXA
Gene Alias
MGC99608, TSD
Gene Description
hexosaminidase A (alpha polypeptide)
Gene Ontology
HyperlinkGene Summary
This gene encodes the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I). [provided by RefSeq
Other Designations
GM2 gangliosidosis|N-acetyl-beta-glucosaminidase|Tay Sachs disease|beta-N-acetylhexosaminidase|hexosaminidase A
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Interactome
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Pathway
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Disease
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